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The Agouti gene controls the distribution of black pigment. The dominant allele A restricts black pigment to the points of the horse (mane, tail, lower legs and ear rims), as seen, for example, in bays and buckskins. The recessive allele a uniformly distributes black pigment over the entire body.
Breeders interested in producing black horses need to have breeding stock carrying the a allele, in addition to the E allele of the Extension gene. Using results from the red factor and agouti tests, click here for a table of breeding schemes to produce black animals.
Agouti results are reported as:
|2 copies of agouti present. If present, black pigment is restricted to the points.|
|A/a||1 copy of agouti. If present, black pigment is restricted to the points.|
|a/a||If present, black pigment is distributed uniformly over the body.|