Exon annotations are from the March 2008 Ensembl gene build (Release 49).
Promoter regions are definied as the 1500 bp interval upstream of the first annotated exon.
ECR positions were obtained from ECRbase (http://ecrbase.dcode.org/) and described in:
G.G. Loots and I. Ovcharenko ECRbase: Database of Evolutionary Conserved Regions, Promoters, and Transcription Factor Binding Sites in Vertebrate Genomes Bioinformatics, 23(1):122-4 (2007)
Ultra-ECRs were previously described in:
Bejerano G, Pheasant M, Makunin I, Stephen S, Kent WJ, Mattick JS, Haussler D. Ultraconserved Elements in the Human Genome. Science, 304(5675), pp. 1321-1325 (2004).
|Product Size:||300-600 bp|
|Primer Size (min)||19|
|Primer Size (opt)||20|
|Primer Size (max)||22|
The T3 tail sequence is appended to the 5' end of the forward primer and T7 sequence is appended to the 5' end of the reverse primer. This affords sequencing independent PCR products with conventional sequencing primers for high-throughput.
T3: AACCCTCACTAAAGGGA T7: TACGACTCACTATAGGG