UC Davis School of Veterinary Medicine Veterinary Genetics Laboratory

Connemara Pony Hoof Wall Separation Disease


Hoof Wall Separation Disease (HWSD) is a genetic defect characterized by a hoof wall that easily breaks and cracks, and a normal appearing coronary band. The breaks and cracks begin to occur in young ponies. In severe cases the pony bears weight entirely on the sole of the foot which can lead to severe lameness. HWSD is inherited as an autosomal recessive trait. This means that carriers are completely normal and only animals with two copies of the mutation will show clinical signs of the disease. HWSD appears to occur only in the Connemara pony and the carrier frequency has been estimated to be about 14.8%. A DNA test for this specific mutation can determine if ponies are normal or if they carry one or two copies of the mutation. Ponies that carry two copies of the mutation are highly likely to be affected with the disease. Some cases are milder while others are more severe. We have identified at least one case where the pony does not appear to have clinical signs associated with having two copies of the mutation. This indicates that the mutation is not fully penetrant; however it has very high penetrance of 96.8%.

The VGL offers a DNA test for HWSD to assist owners and breeders in identifying affected and carrier ponies. The test uses DNA collected from mane hair thus avoiding invasive blood collection. Breeders can use results from the test as a tool for selection of mating pairs to avoid producing affected foals.

Allow 2-6 business days for results.

Results reported as:

N/N: No copies of HWSD mutation; animal is normal

N/HWSD: 1 copy of HWSD mutation; animal is normal but is a carrier

HWSD/HWSD: 2 copies of HWSD mutation; animal is affected

Ponies that have only one mutant copy of HWSD (N/HWSD) are normal but they are carriers of the disease. When two carriers are bred to each other the resulting foals can be affected. At the time that this test was released, approximately 15% of Connemara were carriers of HWSD (N/HWSD) however, the number of carriers can change with each generation. Ponies that are carriers of HWSD are completely normal and they can be safely bred to non-carrier, normal ponies (N/N) in order to maintain diversity within the breed and to select for other positive attributes in carriers.


Manuscript in preparation. Causative mutation identified by Dr. Danika Bannasch and collaborators, Department of Population Health and Reproduction, School of Veterinary Medicine, UC Davis.

Veterinary Genetics Laboratory, Tel 530-752-2211, Email VGL